"Hey guys, here's this awesome new algorithm for label free analysis!!!!"
Want a GREAT dataset to test it out on?
Check out this cool new paper from Claire Ramus et al.,! In this, they developed a great standard that all of us can download and use for free. They also use it to test a bunch of the current algorithms people are using.
The sample is the Sigma UPS1 48 protein mix (all equimolar proteins) spiked into a Yeast digest background at different concentrations. The spike-ins range from 50amol to 12,500 amol (yeah...there is a more efficient way of writing this, but I'm not great at metrics...thanks American public schools! I love sounding like an idiot to everyone else in the world...)
You can download the dataset...wow...my internet is slow right now...well, I guess you can download the dataset...from PRIDE. It will be PXD0001819 after it is published. The authors were kind enough to provide a way to download the dataset prior to publication (wow, right!??!) that you can find in the abstract.
Got a couple files. This study was on an Orbitrap Velos running High-Low (FT for MS1 and CID ion trap MS/MS scans). It looks like 60k resolution at the MS1 (around 400m/z)
Hiç yorum yok:
Yorum Gönder